Wednesday, November 24, 2010

DNA Fingerprinting Virtual Lab

1. DNA is unique for everyone. The only exception is if a person has what?
If the person has a twin.


2. What are DNA fingerprints used for?
Anything from determining a biological mother or father to identifying the suspect of a crime.

Part 1 “It Takes a Lickin”

3. What “crime” was committed?
Jimmy's holographic Nova lolipop.

4. What bodily fluid was removed from the “crime scene” to get DNA?
Spit and fingerprints.

Part 2 “DNA Fingerprinting at the NOVA Lab”


5. What does a restriction enzyme do?
Restriction enzymes act like scissors, cutting the long DNA molecules at different locations.

6. What is agarose gel?
Agarose gel is a thick, porous like substance. It will act as a molecular strainer, allowing smaller pieces of DNA to move through more through more easily than larger pieces.

7. What is electrophoresis?
It is the process of moving molecules with an electric current.

8. Smaller fragments of DNA move ____________ than longer strands?
They move more easily.

9. Why do you need to place a nylon membrane over the gel?
The aragose gel is to hard to work with.

10. Probes attach themselves to __________
They attach themselves to DNA fragments on the nylon membrane.

11. Which chemical in your “virtual lab” is radioactive?
The probes are radioactive.

12. Sketch your DNA fingerprint.
It looks like small bars evenly separated on top of each other. They all have different thicknesses.


13. Based on your DNA fingerprint, who licked the lollipop?

Honey was the culprit.



14. What kinds of things could you do at the DNA workshop?
Learn about protein synthesis and DNA replication.
15. Read an article about genetics at this site that you might find interesting, or use the "Search" box in the upper right hand corner to search for DNA fingerprinting.
Title of Article ____________________________ Author and Date ______________________________________
Summarize what the article was about. Write this in a paragraph format.

DNA fingerprinting

The Columbia Encyclopedia, Sixth Edition | 2008 |
This article DNA. It is also used by law enforcement to identify suspects from hair, blood, semen, or other biological materials found at the scene of a violent crime.A common procedure for DNA fingerprinting is restriction fragment length polymorphism (RFLP). In this method, DNA is extracted from a sample and cut into segments using special restriction enzymes. RFLP focuses on segments that contain sequences of repeated DNA bases, which vary widely from person to person. The segments are separated using a laboratory technique